Recombinant Mouse GM-CSF/CSF2 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSM041032-10ug | 10ug | £219.00 |
Quantity:
| PKSM041032-50ug | 50ug | £497.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Store at < -20°C stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
GM-CSF;CSF2
Accession:
P01587
Activity:
Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 40-170 pg/ml.
Background:
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
Calculated MW:
15.1 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
18-30 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ala18-Lys141
Target Synonym:
Granulocyte-Macrophage Colony-Stimulating Factor;GM-CSF;Colony-Stimulating Factor;CSF;Molgramostin;Sargramostim;CSF2;GMCSF
UNIProt ID:
P01587