Recombinant Human CD40L/TNFSF5 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031976-100ug | 100ug | £615.00 |
Quantity:
| PKSH031976-1mg | 1mg | £3,864.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD40L;TNFSF5
Accession:
NP_000065.1
Activity:
1. Immobilized human CD40 at 2 ug/ml (100 ul/well) can bind biotinylated human CD40L with a linear range of 15.6-500 ng/ml.
2. Immobilized human CD40 / Fc at 2 ug/ml (100 ul/well) can bind biotinylated human CD40L with a linear range of 7.8-125 ng/ml.
Background:
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
Calculated MW:
17.7 kDa
Expression Host:
E.coli
Formulation:
Lyophilized from sterile PBS, pH 7.4, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
18 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Glu 108-Leu 261
Target Synonym:
CD40 Ligand;CD40-L;T-Cell Antigen Gp39;TNF-Related Activation Protein;TRAP;Tumor Necrosis Factor Ligand Superfamily Member 5;CD154;CD40LG;CD40L;TNFSF5;TRAP;hCD40L;HIGM1;IGM;IMD3;T-BAM;TNFSF5;TRAP