Recombinant Human RANKL/TNFSF11 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH032986-20ug | 20ug | £210.00 |
Quantity:
| PKSH032986-100ug | 100ug | £472.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
RANKL;TNFSF11
Accession:
O14788
Activity:
Immobilized ZRKLA at 2ug/ml(100 ul/well) can bind Human OPG-His(Cat: PKSH033573) The ED50 of ZRKLA is 0.15 ug/ml.
Background:
CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.
Calculated MW:
22.4 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM Tris,150mM NaCl,pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-6His
ObservedMW:
20 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Ile140-Asp317
Target Synonym:
CD254;ODF;OPGL;RANKL;TNFSF11;CD254;Osteoclast differentiation factor;Receptor activator of nuclear factor kappa-B ligand;tumor necrosis factor ligand superfamily member 11;hRANKL2;OPTB2;RANKL;sOdf
UNIProt ID:
O14788