Recombinant Human LIF Protein (E.coli)(Active)
| Code | Size | Price |
|---|
| PKSH032694-20ug | 20ug | £210.00 |
Quantity:
| PKSH032694-100ug | 100ug | £472.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
LIF
Accession:
P15018
Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 32.1 pg/ml.
Background:
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Calculated MW:
19.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
18 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ser23-Phe202
Target Synonym:
Leukemia Inhibitory Factor;LIF;Differentiation-Stimulating Factor;D Factor;Melanoma-Derived LPL Inhibitor;MLPLI;Emfilermin;LIF;HILDA;CDF;DIA
UNIProt ID:
P15018