Recombinant Human Interleukin-5/IL-5 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH032653-10ug | 10ug | £219.00 |
Quantity:
| PKSH032653-50ug | 50ug | £497.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
Interleukin-5;IL-5
Accession:
P05113
Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml.
Background:
IL-5 is expressed in eosinophils, NK cells, TC2 CD8+ T cells, mast cells, CD45+ CD4+ T cells, gamma delta T cells and IL-1 beta activated endothelial cells. IL-5 acts as a growth and differentiation factor for both B cells and eosinophils. Relative to B cells, IL-5 appears to induce the differentiation of activated conventional B-2 cells into Ig-secreting cells. In addition, it induces the growth of B-1 progenitors as well as IgM production by B-1 cells.IL-5 appears to perform a number of functions on eosinophils. These include the down modulation of Mac-1,the upregulation of receptors for IgA and IgG,the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release.IL-5 also promotes the growth and differentiation of eosinophils.
Calculated MW:
14.2 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
20 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ile20-Ser134
Target Synonym:
Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5
UNIProt ID:
P05113