Recombinant Human IGF-1/IGF1 Protein (aa 52-118)(Active)
| Code | Size | Price |
|---|
| PKSH032595-10ug | 10ug | £101.00 |
Quantity:
| PKSH032595-50ug | 50ug | £121.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
IGF-1;IGF1
Accession:
P05019
Activity:
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 52. 4 ng/ml.
Background:
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.
Calculated MW:
7.3 kDa
Endotoxin:
< 0.5 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 300mM NaAc, pH 6.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
9 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Thr52-Ala118
Target Synonym:
Insulin-Like Growth Factor I;IGF-I;Mechano Growth Factor;MGF;Somatomedin-C;IGF1;IBP1
UNIProt ID:
P05019