Recombinant Human CD16a/FCGR3A Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH032419-10ug | 10ug | £155.00 |
Quantity:
| PKSH032419-50ug | 50ug | £287.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
CD16a;FCGR3A
Accession:
P08637
Activity:
Immobilized Human IgG1Fc(Cat: PKSH032558) at 10ug/ml(100 ul/well) can bind Human CD16a-His. The ED50 of Human CD16a-His is 2. 57 ug/ml.
Background:
Receptors for the Fc region of immunoglobin G (FcγR) are divided into three classes and FcγRIII is a multifunctional; low/intermediate affinity receptor. In humans; FcγRIII is expressed as two distinct forms (FcγRIIIA and FcγRIIIB) that are encoded by two different but highly homologous genes in a cell type-specific manner. FcγRIIIB is a low-affinity; GPI-linked receptor expressed by neutrophils and eosinophils; whereas FcγRIIIA is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes; natural killer (NK) cells; monocytes; and macrophages. The FcγRIIIA receptor is involved in phagocytosis; secretion of enzymes; inflammatory mediators; antibody-dependent cellular cytotoxicity (ADCC); mast cell degranulation; and clearance of immune complexes. FcγRIIIA has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections; systemic lupus erythematosus; and alloimmune neonatal neutropenia. In humans; it is a 50 -70 kD type I transmembrane activating receptor. The FcγRIIIA cDNA encodes 254 amino acid including a 16aa signal sequence; 191 amino acid ECD with two C2-type Ig-like domains; five potential N-glycosylation sites; a 22 amino acid transmembrane sequence and a 25 amino acid cytoplasmic domain.
Calculated MW:
22.6 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
40 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Gly17-Gln208
Target Synonym:
Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A;CD16a Antigen;Fc-Gamma RIII-Alpha;Fc-Gamma RIII;Fc-gamma RIIIa;FcRIII;FcRIIIa;FcR-10;IgG Fc Receptor III-2;CD16a;FCGR3A;CD16A;FCG3;FCGR3;IGFR3;CD16;CD16A
UNIProt ID:
P08637