Recombinant Human DKK1/Dkk-1 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031807-20ug | 20ug | £320.00 |
Quantity:
| PKSH031807-100ug | 100ug | £587.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
The product is shipped at ambient temperature. Upon receipt store it immediately at the storage temperature listed.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
DKK1;Dkk-1
Accession:
NP_03637°C.1
Activity:
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a.
Background:
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Calculated MW:
25.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
45 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Met 2-His 266
Target Synonym:
Dickkopf-related protein 1;Dickkopf-1;Dkk-1;SK