Recombinant Human CD40L/TNFSF5 Protein (Human Cells)(Active)
| Code | Size | Price |
|---|
| PKSH032214-10ug | 10ug | £451.00 |
Quantity:
| PKSH032214-50ug | 50ug | £595.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD40L;TNFSF5
Accession:
P29965
Activity:
Immobilied Anti-Human CD40L mAb at 5 ug/ml (100 ul/well) can bind Human CD40L. The EC50 of Human CD40L is not higher than 10 ng/ml.
Background:
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members; CD40LG exists as a trimer in membrane bound and soluble form; both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2.
Calculated MW:
16.2 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
16-18 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 113-Leu261
Target Synonym:
CD40 Ligand;CD40-L;T-Cell Antigen Gp39;TNF-Related Activation Protein;TRAP;Tumor Necrosis Factor Ligand Superfamily Member 5;CD154;CD40LG;CD40L;TNFSF5;TRAP;hCD40L;HIGM1;IGM;IMD3;T-BAM;TNFSF5;TRAP
UNIProt ID:
P29965