Recombinant Human CD32a/FCGR2A Protein (167 Arg, Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSH030298-100ug | 100ug | £945.00 |
Quantity:
| PKSH030298-1mg | 1mg | £5,075.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD32a;FCGR2A
Accession:
AAA35827.1
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CD32a-Fc at 10 ug/ml (100 ul/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 ug/ml.
Background:
Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a, FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production.
Calculated MW:
47.3 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-hFc
ObservedMW:
55 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ile 218
Target Synonym:
Low affinity immunoglobulin gamma Fc region receptor II-a;IgG Fc receptor II-a;CDw32;Fc-gamma RII-a;Fc-gamma-RIIa;FcRII-a;CD32;FCGR2A;FCG2;FCGR2A1;IGFR2;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR5