Recombinant Human CD27/TNFRSF7 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031925-100ug | 100ug | £1,003.00 |
Quantity:
| PKSH031925-1mg | 1mg | £3,868.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD27;TNFRSF7
Accession:
P26842
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CD27-his at 10 ug/mL (100 ul/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 57-85 ng/mL.
Background:
CD27; also known as TNFRSF7; is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage; and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer; CD27 has been shown to play roles in lymphoid proliferation; differentiation; and apoptosis.It has important role in generation of T cell immunity; and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule; the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity; and meanwhile; plays a key role in regulating B-cell differentiation; activation and immunoglobulin synthesis.
Calculated MW:
20.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
Baculovirus-Insect Cells
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
27 kDa
Purity:
> 85 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ile192
Target Synonym:
S152;S152. LPFS2;S152.LPFS2;T14;TNFRSF7;Tp55
UNIProt ID:
P26842