Recombinant Human CD16a/FCGR3A Protein (176 Phe, His&AVI Tag)(Active)
| Code | Size | Price |
|---|
| PKSH030285-100ug | 100ug | £1,043.00 |
Quantity:
| PKSH030285-1mg | 1mg | £5,871.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Bio-Layer Interferometry
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD16a;FCGR3A
Accession:
P08637-1
Activity:
Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 30nM.
Background:
The Fc receptor with low affinity for IgG (FCGR3; or CD16) is encoded by 2 nearly identical genes; FCGR3A and FCGR3B; resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A; it is also known as CD16a; encodes a transmembrane protein expressed on activated monocytes/macrophages; natural killer (NK) cells; and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells; independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells; independent of antibody ligation.
Calculated MW:
25.1 kDa
Conjugation:
Biotinylated
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.2
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His & AVI
ObservedMW:
46 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Gln 208
Target Synonym:
Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A;CD16a Antigen;Fc-Gamma RIII-Alpha;Fc-Gamma RIII;Fc-gamma RIIIa;FcRIII;FcRIIIa;FcR-10;IgG Fc Receptor III-2;CD16a;FCGR3A;CD16A;FCG3;FCGR3;IGFR3;CD16;CD16A
UNIProt ID:
P08637