Recombinant Human MGMT Protein (His Tag)
| Code | Size | Price |
|---|
| PKSH032031-10ug | 10ug | £568.00 |
Quantity:
| PKSH032031-50ug | 50ug | £749.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
MGMT
Accession:
P16455
Background:
MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
Calculated MW:
23.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of 20mMTris-HCl,1mM EDTA, pH 8.0 .
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-6His
ObservedMW:
25 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Asn207
Target Synonym:
Methylated-DNA--protein-cysteine methyltransferase;6-O-methylguanine-DNAmethyltransferase;O-6-methylguanine-DNA-alkyltransferase;MGMT
UNIProt ID:
P16455