Recombinant Human CD73/NT5E Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH032030-10ug | 10ug | £536.00 |
Quantity:
| PKSH032030-50ug | 50ug | £833.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
The product is shipped on dry ice/blue ice. Upon receipt store it immediately at the storage temperature listed.
Storage:
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Further Information
Abbreviation:
CD73;NT5E
Accession:
P21589
Activity:
Measured by its ability to hydrolyze the 5?-phosphate group from the substrate adenosine-5?-monophosphate (AMP). The specific activity is 8000-24000 pmol/min/ug.
Background:
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Calculated MW:
58.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-6His
ObservedMW:
62 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Trp27-Lys547
Target Synonym:
5'-Nucleotidase;5'-NT;Ecto-5'-Nucleotidase;CD73;NT5E;NT5;NTE;CALJA;CD73;E5NT;eN;eNT;NT
UNIProt ID:
P21589