His-Tag Polyclonal Antibody

Elabscience
Product Code: E-AB-20048
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-20048-30uL30uL£125.00
Quantity:
E-AB-20048-60uL60uL£146.00
Quantity:
E-AB-20048-120uL120uL£196.00
Quantity:
E-AB-20048-200uL200uL£216.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western Blot analysis of 1ug His fusion protein using His-Tag Polyclonal Antibody at dilution of 1:1000.

Western Blot analysis of 1ug His fusion protein using His-Tag Polyclonal Antibody at dilution of 1:1000.

Documents

Further Information

Abbreviation:
His-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-20000
Immunogen:
Synthetic Peptide
Purification method:
Affinity purification
Target Synonym:
6 His epitope tag;Hexa His tag;HHHHHH epitope tag;HHHHHH tag;His tag;Polyhistidine Tag