MBP-Tag Monoclonal Antibody
| Code | Size | Price |
|---|
| E-AB-20013-30uL | 30uL | £125.00 |
Quantity:
| E-AB-20013-60uL | 60uL | £146.00 |
Quantity:
| E-AB-20013-120uL | 120uL | £196.00 |
Quantity:
| E-AB-20013-200uL | 200uL | £216.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: Clone:8K2
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
MBP-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000
Immunogen:
Recombinant Protein
Purification method:
Protein A purification
Target Synonym:
maltose-binding protein;MBP