His-Tag Monoclonal Antibody
| Code | Size | Price |
|---|
| E-AB-20009-30uL | 30uL | £125.00 |
Quantity:
| E-AB-20009-60uL | 60uL | £146.00 |
Quantity:
| E-AB-20009-120uL | 120uL | £196.00 |
Quantity:
| E-AB-20009-200uL | 200uL | £216.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: Clone:5I8
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Further Information
Abbreviation:
His-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Immunogen:
Synthetic Peptide
Purification method:
Protein A purification
Target Synonym:
6 His epitope tag;Hexa His tag;HHHHHH epitope tag;HHHHHH tag;His tag;Polyhistidine Tag
References
- https://www.mdpi.com/2076-393X/10/12/2066