HIF1 bata Monoclonal Antibody

HIF-1 β/ARNT

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

1 mg/mL

Unconjugated

WB 1:1000-2000, IHC 1:100-200

Recombinant Protein

87kDa

Protein A purification

arnT;ARNT protein;ARNT;Aryl hydrocarbon receptor nuclear translocator;bHLHe2;Class E basic helix-loop-helix protein 2;Dioxin receptor;Dioxin receptor nuclear translocator;Drnt;HIF 1 beta;HIF 1beta;HIF-1-beta;HIF1-beta;HIF1B;HIF1beta;Hypoxia Inducible Factor 1;Hypoxia inducible factor 1 beta;Hypoxia-inducible factor 1-beta;nuclear translocator;Tango

P27540