Recombinant Mouse TRAIL R2/TNFRSF10B Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSM040694-100ug | 100ug | £615.00 |
Quantity:
| PKSM040694-1mg | 1mg | £3,588.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Applications:
- Cell Culture
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
TRAIL R2;TNFRSF10B
Accession:
NP_064671.2
Activity:
1. Immobilized mouse TNFRSF10B-His at 10 ug/ml (100 ul/well) can bind biotinylated human TNFSF10, The EC50 of biotinylated human TNFSF10 is 0.16-0.38 ug/ml.
2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 0.5-2 ug/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
Background:
Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
Calculated MW:
15 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
25-35 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ser 177
Target Synonym:
Tumor Necrosis Factor Receptor Superfamily Member 10B;Death Receptor 5;TNF-Related Apoptosis-Inducing Ligand Receptor 2;TRAIL Receptor 2;TRAIL-R2;CD262;TNFRSF10B;DR5;KILLER;TRAILR2;TRICK2;ZTNFR9