Recombinant Mouse SELP/selectin P/P-selectin Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSM040507-100ug | 100ug | £723.00 |
Quantity:
| PKSM040507-1mg | 1mg | £4,283.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Applications:
- Cell Culture
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
SELP;selectin P;P-selectin
Accession:
Q01102
Activity:
Measured by the ability of the immobilized protein to support the adhesion of U937 cells. When 5 x 104 cells/well are added to SELP-coated plates (5 ug/mL and 100 uL/well), approximately > 70% cells will adhere specifically after 60 minutes at 37°C.
Background:
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
Calculated MW:
74 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
116 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met1-Ala709
Target Synonym:
CD62P;GMP-140;Grmp;LECAM3;PADGEM
UNIProt ID:
Q01102