Recombinant Human IL2Ra/CD25 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031814-100ug | 100ug | £761.00 |
Quantity:
| PKSH031814-1mg | 1mg | £4,546.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
IL2Ra;CD25
Accession:
NP_000408.1
Activity:
1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM.
2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75ug/mL.
Background:
CD25 (alpha-chain of IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development is associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of sIL-2Ralpha. In most haematological malignancies, including different types of leukaemias and lymphomas, sIL-2Ralpha has been found to be released directly from the surface of neoplastic cells thus reflecting the tumour bulk, turnover and activity.
Calculated MW:
23.3 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
44 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Cys 213
Target Synonym:
Interleukin-2 receptor subunit alpha;CD25;p55;TAC antigen;IL2-RA;IL-2R subunit alpha;IL-2-RA;IL-2 receptor subunit alpha;IL-2Rα;IDDM10;IL2R;p55;TCGFR