Recombinant Human CD32b/FCGR2B Protein (His &AVI Tag), Biotinylated(Active)
| Code | Size | Price |
|---|
| PKSH031724-20ug | 20ug | £667.00 |
Quantity:
| PKSH031724-100ug | 100ug | £1,432.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
CD32b;FCGR2B
Accession:
NP_001002274.1
Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 ug/ml (100 ul/well) can bind Human CD32b. The EC50 of Human CD32b is 1. 6 - 3. 7 ug/ml. 2. Labeling ratio of biotin to protein: 0.5
Background:
FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.
Calculated MW:
24 kDa
Conjugation:
Biotinylated
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His-AVI
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ala 46-Ile 224
Target Synonym:
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b;IgG Fc Receptor II-b;CDw32;Fc-Gamma RII-b;Fc-Gamma-RIIb;FcRII-b;CD32;FCGR2B;FCG2;IGFR2