Recombinant Human Carbonic Anhydrase 3/CA3 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031566-100ug | 100ug | £615.00 |
Quantity:
| PKSH031566-1mg | 1mg | £4,283.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
Carbonic Anhydrase 3;CA3
Accession:
NP_005172.1
Activity:
Measured by its esterase activity. The specific activity is > 5 pmoles/min/ug.
Background:
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Calculated MW:
30.4 kDa
Expression Host:
E.coli
Formulation:
Lyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
30.4 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Lys 260
Target Synonym:
Carbonic Anhydrase 3;Carbonate Dehydratase III;Carbonic Anhydrase III;CA-III;CA3;Car3