Recombinant Human NKG2D/CD314 Protein (aa 78-216, His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031517-100ug | 100ug | £664.00 |
Quantity:
| PKSH031517-1mg | 1mg | £4,795.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Further Information
Abbreviation:
NKG2D;CD314
Accession:
NP_031386.2
Activity:
1. Immobilized human His-NKG2D (78-216) at 10 ug/ml (100 ul/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 0.04-0.08 ug/ml.
2. Immobilized human His-NKG2D (78-216) at 10 ug/ml (100 ul/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml.
Background:
NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
Calculated MW:
18.4 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
Baculovirus-Insect Cells
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-His
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Phe78-Val216
Target Synonym:
CD314;KLRK1;CD314 antigen;Killer cell lectin-like receptor subfamily K member 1;killer cell lectin-like receptor subfamily K;member 1;KLR;NK cell receptor D;NKG2-D;NKG2-D type II integral membrane protein;NKG2-D-activating NK recepto