SIRT1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-32901
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-32901-20uL20uL£125.00
Quantity:
E-AB-32901-60uL60uL£172.00
Quantity:
E-AB-32901-120uL120uL£237.00
Quantity:
E-AB-32901-200uL200uL£344.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C

Images

1 / 2
Western blot analysis of lysates from 1)Mouse Ovarian granulosa cell , 2)Mouse Ovarian granulosa cell knockdown by SIRT1-siRNA, primary antibody was diluted at 1:1000.
2 / 2
Immunofluorescence analysis of Rat lung tissue using SIRT1 Polyclonal Antibody at dilution of 1:200.
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Western blot analysis of lysates from 1)Mouse Ovarian granulosa cell , 2)Mouse Ovarian granulosa cell knockdown by SIRT1-siRNA, primary antibody was diluted at 1:1000.
Immunofluorescence analysis of Rat lung tissue using SIRT1 Polyclonal Antibody at dilution of 1:200.

Documents

Further Information

Abbreviation:
SIRT1
Background:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins witHuman, Mouseono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
82kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:5000
Immunogen:
Synthesized peptide derived from human SIRT1 around the non-phosphorylation site of Ser47.
ObservedMW:
81kDa-120kDa
Purification method:
Affinity purification
Target Synonym:
75SirT1;hSIR2;hSIRT1;HST2;S. cerevisiae;homolog of;NAD dependent deacetylase sirtuin 1;NAD dependent protein deacetylase sirtuin 1;OTTHUMP00000198111;OTTHUMP00000198112;Regulatory protein SIR2 homolog 1;SIR1;SIR2;SIR2 like 1;SIR2 like protein 1;SIR2;S.cerevisiae;homolog-like 1;SIR2-like protein 1;SIR2ALPHA;SIR2L1;Sirt1;SirtT1 75 kDa fragment;Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);Sirtuin 1;Sirtuin type 1
UNIProt ID:
Q96EB6

References

  • https://link.springer.com/article/10.1007/s12079-022-00703-0