p53RFP Polyclonal Antibody
Code | Size | Price |
---|
E-AB-15118-20uL | 20uL | £125.00 |
Quantity:
E-AB-15118-60uL | 60uL | £172.00 |
Quantity:
E-AB-15118-120uL | 120uL | £237.00 |
Quantity:
E-AB-15118-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Images
Further Information
Abbreviation:
RNF144B
Background:
p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
Buffer:
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Calculated MW:
34kDa
Concentration:
0.4 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:200-1:1000, IHC 1:50-1:200
Immunogen:
Recombinant protein of human RNF144B
Purification method:
Affinity purification
Target Synonym:
bA528A10.3;E3 ubiquitin protein ligase RNF144B;E3 ubiquitin-protein ligase RNF144B;IBR domain containing 2;IBR domain containing protein 2;IBR domain-containing protein 2;IBRDC 2;IBRDC2;KIAA0161;MGC71786;OTTHUMP00000016079;OTTHUMP00000016080;OTTHUMP00000039360;p53 inducible RING finger protein;p53-inducible RING finger protein;p53RFP;PIR2;R144B;Ring finger 144B;RING finger protein 144B;RNF 144B;RNF144B
UNIProt ID:
Q7Z419