Rat NSE(Neuron Specific Enolase) CLIA Kit

Elabscience
Product Code: E-CL-R0047
Product Group: CLIA Kits
Supplier: Elabscience
CodeSizePrice
E-CL-R0047-24T24T£223.00
Quantity:
E-CL-R0047-48T48T£350.00
Quantity:
E-CL-R0047-96T96T£442.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
NSE
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat NSE in samples.No significant cross-reactivity or interference between Rat NSE and analogues was observed
Detection Range:
62.5-4000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
37.5 pg/mL
Target Species:
Rat
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat NSE. You can calculate the concentration of Rat NSE in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P07323
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat NSE concentrations in Serum, plasma and other biological fluids.