Rat HNF1a(Hepatocyte Nuclear Factor 1 Alpha) CLIA Kit
| Code | Size | Price |
|---|
| E-CL-R0342-24T | 24T | £223.00 |
Quantity:
| E-CL-R0342-48T | 48T | £350.00 |
Quantity:
| E-CL-R0342-96T | 96T | £442.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Documents
Further Information
Abbreviation:
HNF1a
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat HNF1a in samples.No significant cross-reactivity or interference between Rat HNF1a and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Rat
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat HNF1a. You can calculate the concentration of Rat HNF1a in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P15257
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat HNF1a concentrations in Serum, plasma and other biological fluids.