Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) CLIA Kit
| Code | Size | Price |
|---|
| E-CL-R0352-24T | 24T | £223.00 |
Quantity:
| E-CL-R0352-48T | 48T | £350.00 |
Quantity:
| E-CL-R0352-96T | 96T | £442.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Documents
Further Information
Abbreviation:
HIF-1α
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat HIF-1α in samples.No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed
Detection Range:
3.13-200 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
1.88 pg/mL
Target Species:
Rat
Target Synonym:
HIF1A;HIF-1A;MOP1;PASD8;bHLHe78;basic helix-loop-helix transcription factor;
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Tissue homogenates,cell lysates and other biological fluids
Type:
Sandwich
UNIProt ID:
O35800
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.