Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-20911-20uL | 20uL | £125.00 |
Quantity:
| E-AB-20911-60uL | 60uL | £172.00 |
Quantity:
| E-AB-20911-120uL | 120uL | £237.00 |
Quantity:
| E-AB-20911-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Monkey
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Further Information
Abbreviation:
IκB-α (phospho Ser32/36)
Background:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
36kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Immunogen:
Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/36
ObservedMW:
34kDa
Purification method:
Affinity purification
Target Synonym:
I kappa B alpha;I-kappa-B-alpha;IkappaBalpha;IkB-alpha;IKBA;IKBA;IKBalpha;MAD 3;MAD3;Major histocompatibility complex enhancer-binding protein MAD3;NF kappa B inhibitor alpha;NF-kappa-B inhibitor alpha;NFKBI;NFKBIA;Nuclear factor of kappa light chain gene enhancer in B cells;Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
UNIProt ID:
P25963
References
- https://www.sciencedirect.com/science/article/pii/S0753332223000823
- https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c04970
- https://www.sciencedirect.com/science/article/pii/S0014299921005859
- https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.14218
- https://journals.sagepub.com/doi/abs/10.1177/0960327121993224
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320209/
- https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.13876