Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody
Code | Size | Price |
---|
E-AB-21121-20uL | 20uL | £125.00 |
Quantity:
E-AB-21121-60uL | 60uL | £172.00 |
Quantity:
E-AB-21121-120uL | 120uL | £237.00 |
Quantity:
E-AB-21121-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Monkey
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Images
Further Information
Abbreviation:
AMPKα1/2 (phospho Thr183/172)
Background:
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
62kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000
Immunogen:
Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172
ObservedMW:
63kDa
Purification method:
Affinity purification
Target Synonym:
PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;
UNIProt ID:
Q13131,P54646
References
- https://www.mdpi.com/1960060
- https://www.mdpi.com/1907994