P53 Polyclonal Antibody

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Elabscience
Product Code: E-AB-32469
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-32469-20uL20uL£125.00
Quantity:
E-AB-32469-60uL60uL£172.00
Quantity:
E-AB-32469-120uL120uL£237.00
Quantity:
E-AB-32469-200uL200uL£344.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C

Images

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Western Blot analysis of HepG2 cells using p53 Polyclonal Antibody at dilution of 1:1000.
2 / 2
Immunohistochemistry analysis of paraffin-embedded human Colon cancer using p53 Polyclonal Antibody at dilution of 1:200(4? overnight).
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Western Blot analysis of HepG2 cells using p53 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human Colon cancer using p53 Polyclonal Antibody at dilution of 1:200(4? overnight).

Documents

Further Information

Abbreviation:
p53
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
44kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Immunogen:
Synthesized peptide derived from the C-terminal region of human p53
ObservedMW:
43kDa
Purification method:
Affinity purification
Target Synonym:
Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;Tumor protein 53;Tumor protein p53;Tumor suppressor p53
UNIProt ID:
P04637

References

  • https://www.sciencedirect.com/science/article/pii/S0300483X18303937
  • https://www.sciencedirect.com/science/article/pii/S0006291X19321874