NFκB-p65 Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-32233-20uL | 20uL | £125.00 |
Quantity:
| E-AB-32233-60uL | 60uL | £172.00 |
Quantity:
| E-AB-32233-120uL | 120uL | £237.00 |
Quantity:
| E-AB-32233-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Further Information
Abbreviation:
NFκB-p65
Background:
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
60kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000
Immunogen:
Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281.
ObservedMW:
60kDa
Purification method:
Affinity purification
Target Synonym:
Avian reticuloendotheliosis viral (v rel) oncogene homolog A;MGC131774;NF kappa B p65delta3;NFKB3;Nuclear Factor NF Kappa B p65 Subunit;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B cells 3;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;OTTHUMP00000233473;OTTHUMP00000233474;OTTHUMP00000233475;OTTHUMP00000233476;OTTHUMP00000233900;p65;p65 NF kappaB;p65 NFkB;relA;TF65;Transcription factor p65;v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65));V rel avian reticuloendotheliosis viral oncogene homolog A;v rel reticuloendotheliosis viral oncogene homolog A (avian);V rel reticuloendotheliosis viral oncogene homolog A;nuclear factor of kappa light polypeptide gene enhancer in B cells 3;p65
UNIProt ID:
Q04206
References
- https://www.sciencedirect.com/science/article/pii/S0144861722009523
- https://www.sciencedirect.com/science/article/pii/S0753332223000823
- https://journals.lww.com/cmj/Fulltext/9900/Human_papillomavirus,_tobacco,_and_poor_oral.47.aspx
- https://www.sciencedirect.com/science/article/pii/S0006295221003336
- https://europepmc.org/article/pmc/pmc8333701?javascript_support=no
- https://www.sciencedirect.com/science/article/pii/S240584401936181X