Elabscience

Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) CLIA Kit

Product Code:
 
E-CL-M0431
Product Group:
 
CLIA Kits
Supplier:
 
Elabscience
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Chemiluminescent Immunoassay (CLIA)
Shipping:
 
Ice packs
Storage:
 
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
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Standard curve
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Standard curve

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E-CL-M0431-24T24T£223.00
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Further Information

Abbreviation:
NOS2;iNOS
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Mouse NOS2/iNOS in samples.No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed
Detection Range:
62.5-4000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
37.5 pg/mL
Target Species:
Mouse
Target Synonym:
NOS2A;HEP-NOS;I-NOS;
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P29477
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in Serum, plasma and other biological fluids.

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