Human NPHN(Nephrin) CLIA Kit
| Code | Size | Price |
|---|
| E-CL-H1175-24T | 24T | £223.00 |
Quantity:
| E-CL-H1175-48T | 48T | £350.00 |
Quantity:
| E-CL-H1175-96T | 96T | £442.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Documents
Further Information
Abbreviation:
NPHN
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human NPHN in samples.No significant cross-reactivity or interference between Human NPHN and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Human
Target Synonym:
NPHS1;CNF;Nephrosis 1;Congenital Finnish Type;renal glomerulus-specific cell adhesion receptor;
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NPHN. You can calculate the concentration of Human NPHN in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
O60500
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human NPHN concentrations in Serum, plasma and other biological fluids.