Human mTOR(Mammalian Target of Rapamycin) CLIA Kit
| Code | Size | Price |
|---|
| E-CL-H1050-24T | 24T | £223.00 |
Quantity:
| E-CL-H1050-48T | 48T | £350.00 |
Quantity:
| E-CL-H1050-96T | 96T | £442.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Documents
Further Information
Abbreviation:
mTOR
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human mTOR in samples.No significant cross-reactivity or interference between Human mTOR and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Human
Target Synonym:
FRAP1;RAFT1;RAPT1;Mechanistic target of rapamycin;FK506 binding protein 12-rapamycin associated protein 1;
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human mTOR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human mTOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human mTOR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human mTOR. You can calculate the concentration of Human mTOR in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P42345
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human mTOR concentrations in Serum, plasma and other biological fluids.