GSK3 alpha/beta Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-31628-20uL | 20uL | £125.00 |
Quantity:
| E-AB-31628-60uL | 60uL | £172.00 |
Quantity:
| E-AB-31628-120uL | 120uL | £237.00 |
Quantity:
| E-AB-31628-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Further Information
Abbreviation:
GSK3α/β
Background:
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3βGSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
51kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Immunogen:
Synthesized peptide derived from human GSK3α/β around the non-phosphorylation site of Tyr279/216.
ObservedMW:
51,46kDa
Purification method:
Affinity purification
Target Synonym:
GSK3A;Glycogen synthase kinase-3 alpha;GSK-3 alpha;Serine/threonine-protein kinase GSK3A;GSK3B;Glycogen synthase kinase-3 beta;GSK-3 beta;Serine/threonine-protein kinase GSK3B
UNIProt ID:
P49840,P49841
References
- https://www.frontiersin.org/articles/10.3389/fonc.2019.01469/full