EGFR Polyclonal Antibody

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Elabscience
Product Code: E-AB-31281
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-31281-20uL20uL£125.00
Quantity:
E-AB-31281-60uL60uL£172.00
Quantity:
E-AB-31281-120uL120uL£237.00
Quantity:
E-AB-31281-200uL200uL£344.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C

Images

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Western Blot analysis of 293 cells using EGFR Polyclonal Antibody at dilution of 1:1000.
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Western Blot analysis of 293 cells using EGFR Polyclonal Antibody at dilution of 1:1000.

Documents

Further Information

Abbreviation:
EGFR
Background:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.
Buffer:
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Calculated MW:
134kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:40000
Immunogen:
Synthesized peptide derived from human EGFR around the non-phosphorylation site of Thr693.
ObservedMW:
180,134kDa
Purification method:
Affinity purification
Target Synonym:
Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog);Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian);Epidermal growth factor receptor;erb-b2 receptor tyrosine kinase 1;ERBB;ERBB1;Errp;HER1;mENA;NISBD2;Oncogen ERBB;PIG61;Proto-oncogene c-ErbB-1;Receptor tyrosine protein kinase ErbB 1;Receptor tyrosine-protein kinase ErbB-1;SA7;Species antigen 7;Urogastrone;v-erb-b Avian erythroblastic leukemia viral oncogen homolog;wa2;Wa5
UNIProt ID:
P00533

References

  • https://www.sciencedirect.com/science/article/pii/S0753332220305114