DUT Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-12372-20uL | 20uL | £125.00 |
Quantity:
| E-AB-12372-60uL | 60uL | £172.00 |
Quantity:
| E-AB-12372-120uL | 120uL | £237.00 |
Quantity:
| E-AB-12372-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Further Information
Abbreviation:
DUT
Background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Buffer:
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Calculated MW:
27kDa
Concentration:
0.2 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:200-1:1000, IHC 1:10-1:50
Immunogen:
Synthetic peptide of human DUT
Purification method:
Affinity purification
Target Synonym:
Deoxyuridine 5' triphosphate nucleotidohydrolase;nuclear isoform;Deoxyuridine 5'-triphosphate nucleotidohydrolase;mitochondrial;Deoxyuridine triphosphatase;dut;DUT;dUTP nucleotidohydrolase;dUTP pyrophosphatase;dUTPase;FLJ20622
UNIProt ID:
P33316