ADO Polyclonal Antibody
| Code | Size | Price |
|---|
| E-AB-15460-20uL | 20uL | £125.00 |
Quantity:
| E-AB-15460-60uL | 60uL | £172.00 |
Quantity:
| E-AB-15460-120uL | 120uL | £237.00 |
Quantity:
| E-AB-15460-200uL | 200uL | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ice packs
Storage:
-20°C
Further Information
Abbreviation:
ADO
Background:
Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Buffer:
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Calculated MW:
30kDa
Concentration:
0.3 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:1000-1:5000, IHC 1:25-1:100
Immunogen:
Synthetic peptide of human ADO
Purification method:
Affinity purification
Target Synonym:
2 aminoethanethiol dioxygenase;2-aminoethanethiol dioxygenase;ADO 2 aminoethanethiol (cysteamine) dioxygenase;ADO;AEDO;C10orf22;Chromosome 10 open reasing frame 22;cysteamine (2-aminoethanethiol) dioxygenase (ADO);Cysteamine dioxygenase;DKFZp564C046;FLJ14547
UNIProt ID:
Q96SZ5