Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
| Code | Size | Price |
|---|
| E-EL-R1327-24T | 24T | £207.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-R1327-48T | 48T | £321.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-R1327-96T | 96T | £403.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
CKMB
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Rat
Target Synonym:
CKMB
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P00564(CKM)&P07335(CKB)
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.
References
- https://www.nature.com/articles/s41467-020-20844-3
- https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01639-8
- https://www.hindawi.com/journals/omcl/2022/1090893/
- https://www.sciencedirect.com/science/article/pii/S0753332222008265
- https://www.sciencedirect.com/science/article/pii/S0024320522008232
- https://www.ahajournals.org/doi/abs/10.1161/JAHA.121.023378
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099415/
- https://portlandpress.com/clinsci/article-abstract/130/5/377/77812
- https://www.sciencedirect.com/science/article/pii/S0014299922004332
- https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16491
- https://cardiab.biomedcentral.com/articles/10.1186/s12933-016-0460-z
- https://link.springer.com/article/10.1007/s11356-021-15409-w