Mouse C3a(Complement Component 3a) ELISA Kit
| Code | Size | Price |
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| E-EL-M0337-24T | 24T | £207.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-M0337-96T | 96T | £317.00£403.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-M0337-48T | 48T | £321.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
C3a
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Mouse C3a in samples. No significant cross-reactivity or interference between Mouse C3a and analogues was observed
Detection Range:
0.16-10 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.1 ng/mL
Target Species:
Mouse
Target Synonym:
C3a-desArg;ASP;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P01027
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse C3a concentrations in serum, plasma and other biological fluids.
References
- https://www.karger.com/Article/Abstract/447714
- https://www.sciencedirect.com/science/article/pii/S0147651318301477