Human IL-35(Interleukin 35) ELISA Kit
| Code | Size | Price |
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| E-EL-H2443-24T | 24T | £207.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-H2443-96T | 96T | £317.00£403.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-H2443-48T | 48T | £321.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
IL-35
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed
Detection Range:
15.63-1000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
9.38 pg/mL
Target Species:
Human
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human IL-35. You can calculate the concentration of Human IL-35 in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P29459,Q14213
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids.
References
- https://gut.bmj.com/content/early/2021/08/02/gutjnl-2021-324071.abstract
- https://www.nature.com/articles/s41467-022-28338-0
- https://link.springer.com/article/10.1186/s12974-019-1606-1
- https://www.nature.com/articles/s41598-019-52286-3
- https://www.mdpi.com/688776
- https://link.springer.com/article/10.1007/s13277-015-4616-3
- https://link.springer.com/article/10.1007/s10067-022-06137-8
- https://www.liebertpub.com/doi/abs/10.1089/vim.2020.0176
- https://www.spandidos-publications.com/10.3892/ol.2017.5949
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783395/