Human β-CTx(Beta Crosslaps) ELISA Kit

β-CTx

2.5h

This kit recognizes Human β-CTx in samples.No significant cross-reactivity or interference between Human β-CTx and analogues was observed

125-8000 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

75 pg/mL

Human

b-CTx;bCTXI;bCTX-I;B-Cr;BCL;Type I Collagen C-Telopeptide-Related Fraction;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-CTx. During the reaction, Human β-CTx in the sample or standard competes with a fixed amount of Human β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Human β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Competitive

P02452

This ELISA kit applies to the in vitro quantitative determination of Human β-CTx concentrations in serum, plasma and other biological fluids.