ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit
| Code | Size | Price |
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| E-EL-0033-24T | 24T | £183.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-EL-0033-48T | 48T | £275.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-EL-0033-96T | 96T | £291.00£342.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
ST;5-HT
Assay Time:
2.5h
Cross reactivity:
This kit recognizes Universal ST/5-HT in samples.No significant cross-reactivity or interference between Universal ST/5-HT and analogues was observed
Detection Range:
15.63-1000 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
9.38 ng/mL
Target Species:
Universal
Target Synonym:
5HT;Enteramine;Thrombocytin;Thrombotonin;3-(β-Aminoethyl)-5-hydroxyindole;
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST/5-HT. During the reaction, Universal ST/5-HT in the sample or standard competes with a fixed amount of Universal ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Universal ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Competitive
Usage:
This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids.
References
- https://www.sciencedirect.com/science/article/pii/S0016508517302718
- https://aacrjournals.org/cancerimmunolres/article-abstract/9/9/1008/666146
- https://pubmed.ncbi.nlm.nih.gov/34964929/
- https://www.sciencedirect.com/science/article/pii/S0269749121013968
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555466/
- https://www.sciencedirect.com/science/article/pii/S1043661822000354
- https://www.sciencedirect.com/science/article/pii/S0753332222008265
- https://www.sciencedirect.com/science/article/pii/S0753332222015037
- https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200481
- https://www.mdpi.com/2072-6643/14/19/4083
- https://www.frontiersin.org/articles/10.3389/fcimb.2022.945851/full?utm_source=dlvr.it&utm_medium=twitter
- https://www.sciencedirect.com/science/article/pii/S0753332219327489