Human DHEA-S(Dehydroepiandrosterone Sulfate) ELISA Kit

DHEA-S

2.5h

This kit recognizes Human DHEA-S in samples.No significant cross-reactivity or interference between Human DHEA-S and analogues was observed

15.63-1000 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 ng/mL

Human

Androstenolone sulfate;Prasterone sulfate;Androst-5-en-3β-ol-17-one 3β-sulfate;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Human DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Serum, plasma and other biological fluids

Competitive

This ELISA kit applies to the in vitro quantitative determination of Human DHEA-S concentrations in Serum, plasma and other biological fluids.