Human Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

Elabscience
Product Code: E-EL-H0326
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-EL-H0326-24T24T£183.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-H0326-48T48T£275.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-H0326-96T96T£342.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
Ang-II
Assay Time:
2.5h
Cross reactivity:
This kit recognizes Human Ang-II in samples.No significant cross-reactivity or interference between Human Ang-II and analogues was observed
Detection Range:
31.25-2000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
18.75 pg/mL
Target Species:
Human
Target Synonym:
Angiotensin-2
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-II. During the reaction, Human Ang-II in the sample or standard competes with a fixed amount of Human Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Human Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Competitive
UNIProt ID:
P01019
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human Ang-II concentrations in serum, plasma and other biological fluids.

References

  • https://link.springer.com/article/10.1186/s10194-021-01306-7
  • https://www.mdpi.com/article/10.3390/ijms24010343
  • https://static.frontiersin.org/articles/10.3389/fendo.2022.912064/full
  • https://europepmc.org/article/pmc/pmc8866968?javascript_support=no
  • https://link.springer.com/article/10.1186/s12967-021-02755-z
  • https://www.mdpi.com/1422-0067/21/11/3861
  • https://www.sciencedirect.com/science/article/pii/S0939475322000461
  • https://www.hindawi.com/journals/cmmm/2022/6312100/