GFP Monoclonal Antibody

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Elabscience
Product Code: E-AB-48010
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-48010-20uL20uL£110.00
Quantity:
E-AB-48010-50uL50uL£143.00
Quantity:
E-AB-48010-100uL100uL£177.00
Quantity:
E-AB-48010-200uL200uL£210.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 5B5
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
2 / 2
1x106 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
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Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
1x106 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)

Documents

Further Information

Abbreviation:
GFP-Tag
Background:
The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
32kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:100000-200000; IP: 3ug/sample; IF: 1:8000-16000,FCM:0.2 ug
Immunogen:
Recombinant GFP protein expressed by E.coli
ObservedMW:
32kDa
Purification method:
Protein A/G Purified
Target Synonym:
GFP;Green fluorescent protein
UNIProt ID:
P42212

References

  • https://pubmed.ncbi.nlm.nih.gov/35464458/
  • https://pubmed.ncbi.nlm.nih.gov/36533583/https://pubmed.ncbi.nlm.nih.gov/35227760/
  • https://pubmed.ncbi.nlm.nih.gov/33310083/