TNF-α Polyclonal Antibody

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Elabscience
Product Code: E-AB-68252
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-68252-60uL60uL£210.00
Quantity:
E-AB-68252-120uL120uL£291.00
Quantity:
E-AB-68252-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Polyclonal Antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 4
Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 4
Immunofluorescence analysis of Human mammary cancer cells using TNF-α Polyclonal antibody at dilution of 1:100. Blue:DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Polyclonal Antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Human mammary cancer cells using TNF-α Polyclonal antibody at dilution of 1:100. Blue:DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
TNF-α
Background:
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
25kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human TNF
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha
UNIProt ID:
P01375