NRF2 Polyclonal Antibody

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Elabscience
Product Code: E-AB-68254
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-68254-60uL60uL£210.00
Quantity:
E-AB-68254-120uL120uL£291.00
Quantity:
E-AB-68254-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.HeLa cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
2 / 5
Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
3 / 5
Western blot analysis of various lysates using NRF2 Polyclonal antibody at dilution.C6 cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
4 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 5
Immunohistochemistry of paraffin-embedded human placenta using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.HeLa cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
Western blot analysis of various lysates using NRF2 Polyclonal antibody at dilution.C6 cells were treated by MG132 (50 uM) at 37°C for 90 minutes.
Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
NRF2
Background:
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Buffer:
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Calculated MW:
65kDa/66kDa/67kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human NRF2
ObservedMW:
97-100KDa
Purification method:
Affinity purification
Target Synonym:
NFE2L2;HEBP1;NRF2
UNIProt ID:
Q16236